THE EFFECTS OF SALINITY CONCENTRATION ON AN ENZYME CATALYZED REACTION HELEN prof BUELL BIO 101L-03 11/22/11 The effects of common salt tightness on an enzyme catalyzed reaction INTRODUCTION This examine was performed to investigate how the strong-arm factors of table salt testament affect the roll of an enzyme catalyzed reaction. (How strong an enzyme will turn over a substrate, establish on energy of activation.) We tested on various levels of table salt preoccupation, and used catechol to stage substrate, and white potato vine root for to represent enzyme. We unploughed the levels of the substrate and enzyme at a constant, in an sudor to exactly test on the brininess denseness. If our hypothesis is supported, our results will ver balise that the higher concentration of salt, the faster the enzyme will denature. MATERIALS AND METHODS * 5 mL of potato extract * 10 mL of catechol * 3 mL of .1% salinity concentration * 3 mL of .9% salinity concentration * 3 mL of 2% salinity concentration * 3 mL of 5% salinity concentration * 3 mL of 20% salinity concentration * 1- 5 mL pipettes * Pi-pump for 5 mL pipette * Wax soft touch * 5 test subways * Test resistance rack My teammate and I placed 1 mL of potato extract in five test pipages, on with 2 mL of catechol, to that we added 3 mL of .1% salinity concentration in pipework one, 3 mL of .9% salinity concentration in metro two, 2% salinity concentration in tube three, 3 mL of 5% salinity concentration in tube ! four, and lastly 3 mL of 20% salinity concentration in tube five. Pour each potpourri in its own spec 20 tube, along with a separate spec 20 tube alter with wholly potato extract to use as a keep on the...If you want to get a wide of the memorialize essay, order it on our website: OrderEssay.net
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